Substrate regulation and specificity of amylases fromAureobasidiumstrain NRRL Y-12,974

Substrate specificity and activity regulation of protein kinase MELK.

Maternal embryonic leucine zipper kinase (MELK) is a protein Ser/Thr kinase that has been implicated in stem cell renewal, cell cycle progression, and pre-mRNA splicing, but its substrates and regulation are not yet known. We show here that MELK has a rather broad substrate specificity and does not appear to require a specific sequence surrounding its (auto)phosphorylation sites. We have mapped...

Regulation of CDK7 substrate specificity by MAT1 and TFIIH.

The cyclin-dependent kinase (CDK)-activating kinase CAK has been proposed to function in the control of cell cycle progression, DNA repair and RNA polymerase II (pol II) transcription. Most CAK exists as complexes of three subunits: CDK7, cyclin H (CycH) and MAT1. This tripartite CAK occurs in a free form and in association with 'core' TFIIH, which functions in both pol II transcription and DNA...

Serum amylases, isoenzymes, and pancreatitis. I. Effect of substrate variation.

Four plant starches were used to study human amylase activity in normal serum and urine, “pancreatitis” serum,duodenal fluid (secretin stimulated),saliva, and pancreatic extract. The starches were derived from waxy maize, high amylosecorn, potato, and corn (pearl), and were lintnerized. It was shown that the rate of digestion of starch by each of the fluids tested dependson the plant starch sel...

Hydrogen donor specificity of cobamide-dependent ribonucleotide reductase and allosteric regulation of substrate specificity.

Analysis of substrate specificity and cyclin Y binding of PCTAIRE-1 kinase

PCTAIRE-1 (cyclin-dependent kinase [CDK] 16) is a highly conserved serine/threonine kinase that belongs to the CDK family of protein kinases. Little is known regarding PCTAIRE-1 regulation and function and no robust assay exists to assess PCTAIRE-1 activity mainly due to a lack of information regarding its preferred consensus motif and the lack of bona fide substrates. We used positional scanni...